{"id":199,"date":"2020-10-23T17:38:08","date_gmt":"2020-10-23T17:38:08","guid":{"rendered":"https:\/\/www.laboratoriodiprever.com.br\/?page_id=199"},"modified":"2020-12-07T20:14:23","modified_gmt":"2020-12-07T20:14:23","slug":"imunofluorescencia","status":"publish","type":"page","link":"https:\/\/www.laboratoriodiprever.com.br\/imunofluorescencia\/","title":{"rendered":"Imunofluoresc\u00eancia"},"content":{"rendered":"\t\t
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Imunofluoresc\u00eancia<\/h1>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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O estudo de imunofluoresc\u00eancia consiste na avalia\u00e7\u00e3o minuciosa de ant\u00edgenos teciduais, como as imunoglobulinas humanas e derivados do complemento, os quais t\u00eam sido utilizadas na complementa\u00e7\u00e3o diagn\u00f3stica de v\u00e1rias doen\u00e7as. A t\u00e9cnica pode ser realizada em qualquer tecido, contudo suas aplica\u00e7\u00f5es t\u00eam sido mais amplas na dermatologia e nefrologia. Dentre as principais indica\u00e7\u00f5es, est\u00e3o as doen\u00e7as bolhosas cut\u00e2neas, l\u00fapus eritematoso, glomerulopatias e vasculites.

A imunofluoresc\u00eancia direta \u00e9 uma t\u00e9cnica aplicada em cortes histol\u00f3gicos de tecido fixado em meio de Michel, onde se analisa a presen\u00e7a de IgA, IgM, IgG, fatores C3 e C1q, fibrina, Kappa e\/ou Lambda. Sempre deve ser analisada em conjunto com o exame histopatol\u00f3gico a fim de que possa oferecer um resultado mais definitivo.

Para que este exame possa fornecer o m\u00e1ximo de informa\u00e7\u00f5es e que as mesmas sejam de alta qualidade, \u00e9 imprescind\u00edvel realizar o acondicionamento adequado do material. Junto ao acondicionamento, \u00e9 essencial o contato com o Laborat\u00f3rio DI PREVER antes da coleta, pois, dessa forma, poderemos oferecer um diagn\u00f3stico de imunofluoresc\u00eancia de qualidade. <\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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IMPORTANTE:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\u00b9Para exames realizados atrav\u00e9s de operadoras de sa\u00fade complementar (conv\u00eanios), a guia do pedido dever\u00e1 ser entregue no laborat\u00f3rio e o kit de coleta ser\u00e1 disponibilizado ap\u00f3s a autoriza\u00e7\u00e3o por parte da operadora de sa\u00fade. Este exame est\u00e1 coberto em nosso portf\u00f3lio nas operadoras: UNIMED, SC-SA\u00daDE, PLADISA e SA\u00daDE CONCEI\u00c7\u00c3O.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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IMPORTANTE:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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\u00b9Para exames realizados atrav\u00e9s de operadoras de sa\u00fade complementar (conv\u00eanios), a guia do pedido dever\u00e1 ser entregue no laborat\u00f3rio e o kit de coleta ser\u00e1 disponibilizado ap\u00f3s a autoriza\u00e7\u00e3o por parte da operadora de sa\u00fade. Este exame est\u00e1 coberto em nosso portf\u00f3lio nas operadoras: UNIMED, SC-SA\u00daDE, PLADISA e SA\u00daDE CONCEI\u00c7\u00c3O.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t

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Acondicionamento\/ Orienta\u00e7\u00f5es:<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

1. Solicitar ao Laborat\u00f3rio DI PREVER o kit de coleta de estudos de imunofluoresc\u00eancia\u00b9 , o qual ir\u00e1 conter 1 frasco de meio de Michel e 1 frasco de formalina tamponada a 10%;
2. Preencher o pedido m\u00e9dico por completo, especialmente o campo de hip\u00f3teses diagn\u00f3sticas, assim como assinalar quais testes devem ser realizados (nesse caso, sempre marcar os itens anatomopatol\u00f3gico e imunofluoresc\u00eancia);
3. Coletar, pelo menos, 2 amostras, as quais seguir\u00e3o fluxos distintos de an\u00e1lise:
3.1. Amostra 1 ser\u00e1 acondicionada, imediatamente, ap\u00f3s a coleta no frasco do meio de Michel e deve-se certificar que o material esteja totalmente envolvido pela solu\u00e7\u00e3o fixadora (esta amostra jamais deve entrar em contato com solu\u00e7\u00e3o de formalina);
3.2. Amostra 2 ser\u00e1 acondicionada, imediatamente, ap\u00f3s a coleta no frasco da formalina tamponada a 10% e deve-se certificar que o material esteja totalmente envolvido pela solu\u00e7\u00e3o fixadora.
4. As amostras coletadas dever\u00e3o ser encaminhadas \u00e0s unidades do laborat\u00f3rio DI PREVER, imediatamente, ap\u00f3s a sua coleta e recomendamos que elas ocorram, preferencialmente, de segunda a quarta-feira, no per\u00edodo da manh\u00e3;
5. Ap\u00f3s o cadastramento, ser\u00e1 disponibilizado 2 protocolos de exames (1 para o anatomopatol\u00f3gico e 1 para a imunofluoresc\u00eancia), cujo resultado poder\u00e1 ser adquirido em nossas unidades, atrav\u00e9s de nosso site ou de nossos canais digitais de atendimento.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Refer\u00eancias<\/a>\n\t\t\t\t\t<\/div>\n\n\t\t\t\t\t

AOKI, Val\u00e9ria et al. Imunofluoresc\u00eancia direta e indireta. Anais Brasileiros de Dermatologia<\/strong>, v. 85, n. 4, p. 490-500, 2010. Dispon\u00edvel em: https:\/\/www.scielo.br\/pdf\/abd\/v85n4\/v85n4a10.pdf. Acesso em: 20 jul. 2020.<\/p>

SHETTY, Varsha M.; SUBRAMANIAM, Kumudhini; RAO, Raghavendra. Utility of immunofluorescence in dermatology. Indian Dermatology Online Journal<\/strong>, v. 8, n. 1, p. 1-8, January February. 2017. DOI: 10.4103\/2229-5178.198774. Dispon\u00edvel em: http:\/\/www.idoj.in\/article.asp?issn=2229-5178;year=2017;volume=8;issue=1;spage=1;epage=8;aulast=Shetty. Acesso em: 20 jul. 2020.<\/p>

SINHA, Preema et al. Analysis of the utility of direct immunofluorescence in the diagnosis of common immune mediated dermatological conditions. Journal of Marine Medical Society<\/strong>, v. 22, n. 1, p. 44-49, January-June 2020. DOI: 10.4103\/jmms.jmms_15_19. Dispon\u00edvel em: http:\/\/www.marinemedicalsociety.in\/downloadpdf.asp?issn=0975-3605;year=2020;volume=22;issue=1;spage=44;epage=49;aulast=Sinha;type=2. Acesso em: 20 jul. 2020.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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FLUXOGRAMA<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Imunofluoresc\u00eancia Conhe\u00e7a o Exame O estudo de imunofluoresc\u00eancia consiste na avalia\u00e7\u00e3o minuciosa de ant\u00edgenos teciduais, como as imunoglobulinas humanas e derivados do complemento, os quais t\u00eam sido utilizadas na complementa\u00e7\u00e3o diagn\u00f3stica de v\u00e1rias doen\u00e7as. A t\u00e9cnica pode ser realizada em qualquer tecido, contudo suas aplica\u00e7\u00f5es t\u00eam sido mais amplas na dermatologia e nefrologia. Dentre as […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"footnotes":""},"yoast_head":"\nImunofluoresc\u00eancia - Laborat\u00f3rio Di Prever<\/title>\n<meta name=\"description\" content=\"Fa\u00e7a seus exames de Imunofluoresc\u00eancia no Laborat\u00f3rio Di Prever. 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